Human IGFBP3 Recombinant Protein (N-His) (HB776011)
概述
图片
参考文献
产品说明书
概述
货号
HB776011
品牌
abinScience
描述
Recombinant Human IGFBP3 Protein, N-His (HB776011) expressed in Mammalian Cells, spanning Gly28-Lys291. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P17936
蛋白长度
Gly28-Lys291
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Remove endotoxin and sterilization is required for cell assay.
纯度
>90% as determined by SDS-PAGE.
预测分子量
32.68 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
IBP-3, IBP3, IGF-binding protein 3, IGFBP-3, IGFBP3, Insulin-like growth factor-binding protein 3
背景
Insulin-like growth factor-binding protein 3 is a ~31 kDa protein. Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1.
1. Liu, B. et al. (2000) The Journal of biological chemistry 275, 33607-13. PMID: 10874028
3. Ingermann, AR. et al. (2010) The Journal of biological chemistry 285, 30233-46. PMID: 20353938
4. Muzumdar, RH. et al. (2009) PloS one 4, e6334. PMID: 19623253
5. Lue, Y. et al. (2010) Endocrinology 151, 350-7. PMID: 19952275
6. Leal, SM. et al. (1997) The Journal of biological chemistry 272, 20572-6. PMID: 9252371
7. Huang, SS. et al. (2004) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 18, 1719-21. PMID: 15371331
