Recombinant Human ENPP7 Protein, C-Fc (HD356011) expressed in Mammalian Cells, spanning Met1-Pro421. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
Q6UWV6
蛋白长度
Met1-Pro421
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
74.07 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Alk-SMase, Alkaline sphingomyelin phosphodiesterase, E-NPP 7, EC:3.1.4.12, ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, Intestinal alkaline sphingomyelinase, NPP-7
背景
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 is a ~51 kDa protein. Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Also has phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen.
1. Duan, RD. et al. (2003) Journal of lipid research 44, 1241-50. PMID: 12671034
2. Duan, RD. et al. (2003) The Journal of biological chemistry 278, 38528-36. PMID: 12885774
3. Wu, J. et al. (2004) American journal of physiology. Gastrointestinal and liver physiology 287, G967-73. PMID: 15205117
4. Wu, J. et al. (2006) The Biochemical journal 394, 299-308. PMID: 16255717
5. Gorelik, A. et al. (2017) The Journal of biological chemistry 292, 7087-7094. PMID: 28292932
