Recombinant Human CFB Protein, C-His (HF634011) expressed in Mammalian Cells, spanning Met1-Leu764. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P00751
蛋白长度
Met1-Leu764
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
86.50 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
BF, BFD, Ba, Bb, C3/C5 convertase, CFB, Complement factor B, Complement factor B Ba, Complement factor B Bb, EC:3.4.21.47, GBG, Glycine-rich beta glycoprotein, PBF2, Properdin factor B
背景
Complement factor B is a ~85 kDa protein. Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases. CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases.
1. Pangburn, MK. et al. (1986) The Biochemical journal 235, 723-30. PMID: 3638964
2. Vogt, W. et al. (1978) Immunology 34, 29-40. PMID: 624565
3. DiScipio, RG. et al. (1983) The Journal of biological chemistry 258, 10629-36. PMID: 6554279
4. Vogel, CW. et al. (1982) The Journal of biological chemistry 257, 8292-9. PMID: 6919543
5. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277
