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Yersinia pestis Plasminogen activator/Pla Recombinant Protein (N-His) (JN173022)

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概述
货号JN173022
品牌abinScience
描述
Recombinant Yersinia pestis Plasminogen activator/Pla Protein, N-His (JN173022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
表达系统E. coli
Accession号P17811
蛋白长度Ala21-Phe312
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Yersinia pestis
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Coagulase/fibrinolysin, EC:3.4.23.48, Pla, Plasminogen activator, pla
背景

Plasminogen activator is a ~34 kDa protein. In the mammalian host activates (cleaves) plasminogen to generate the serine protease plasmin. Plasmin degrades fibrin clots (fibrinolysis) and facilitates bacterial cell migration, enabling rapid dissemination of bacteria from the initial site of infection (Probable). Cleaves host plasminogen to generate plasmin and probably also has autocatalytic activity. Fibrinolytic activity prevails at 37 degrees Celsius whereas coagulase expression predominates at lower temperatures (28 degrees Celsius). Cleaves plasminogen; plasminogen cleavage is much higher than coagulase activity.

1. Eren, E. et al. (2010) Structure (London, England : 1993) 18, 809-18. PMID: 20637417
2. Eren, E. et al. (2012) The Journal of biological chemistry 287, 23971-6. PMID: 22645135
3. McDonough, KA. et al. (1989) Molecular microbiology 3, 767-75. PMID: 2526282
4. Sodeinde, OA. et al. (1989) Infection and immunity 57, 1517-23. PMID: 2651310
NoteFor research use only
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参考文献
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公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
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体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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