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Human RPE65 Recombinant Protein (N-His) (HD563012)

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概述
货号HD563012
品牌abinScience
描述
Recombinant Human RPE65 Protein, N-His (HD563012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
表达系统E. coli
Accession号Q16518
蛋白长度Met1-Ser533
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名All-trans-retinyl-palmitate hydrolase, EC:3.1.1.64, EC:5.3.3.22, Lutein isomerase, Meso-zeaxanthin isomerase, RPE65, Retinal pigment epithelium-specific 65 kDa protein, Retinoid isomerohydrolase, Retinol isomerase
背景

Retinoid isomerohydrolase is a ~60 kDa protein. Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester.

1. Moiseyev, G. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 12413-8. PMID: 16116091
2. Jacobson, SG. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 15123-8. PMID: 17848510
3. Shyam, R. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 10882-10887. PMID: 28874556
NoteFor research use only
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公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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Human RPE65 Recombinant Protein (N-His)

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