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Human ITSN1 Recombinant Protein (N-His) (HD585012)

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概述
货号HD585012
品牌abinScience
描述
Recombinant Human ITSN1 Protein, N-His (HD585012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
表达系统E. coli
Accession号Q15811
蛋白长度Asp1216-Leu1445
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名ITSN, ITSN1, Intersectin-1, SH3 domain-containing protein 1A, SH3D1A, SH3P17
背景

Intersectin-1 is a ~195 kDa protein. Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery. Acts as a guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex. Plays a role in the assembly and maturation of clathrin-coated vesicles. Recruits FCHSD2 to clathrin-coated pits. Involved in endocytosis of activated EGFR, and probably also other growth factor receptors.

1. Hussain, NK. et al. (2001) Nature cell biology 3, 927-32. PMID: 11584276
2. Almeida-Souza, L. et al. (2018) Cell 174, 325-337.e14. PMID: 29887380
3. Teckchandani, A. et al. (2012) Molecular biology of the cell 23, 2905-16. PMID: 22648170
4. Jenna, S. et al. (2002) The Journal of biological chemistry 277, 6366-73. PMID: 11744688
NoteFor research use only
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参考文献
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公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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