Recombinant Human FAAH Protein, N-His (HD694012). Purity: >90% as determined by SDS-PAGE..
Highlights
●High Purity — >90% as determined by SDS-PAGE.
表达系统
E. coli
Accession号
O00519
蛋白长度
Ser71-Ser579
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fatty-acid amide hydrolase 1 is a ~63 kDa protein. Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids.
1. Wei, BQ. et al. (2006) The Journal of biological chemistry 281, 36569-78. PMID: 17015445
2. Kaczocha, M. et al. (2010) The Journal of biological chemistry 285, 2796-806. PMID: 19926788
3. Giang, DK. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 2238-42. PMID: 9122178
4. Xie, S. et al. (2010) Chemical research in toxicology 23, 1890-904. PMID: 21049984
