GMP Grade Human IL-3 (Tag Free) (HY262021) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
●Recombinant — Consistent lot-to-lot performance.
●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
●Bioactive — Verified biological activity.
表达系统
Mammalian cells
Accession号
P08700
蛋白长度
Ala20-Phe152
应用
ELISA, Functional assay
种属
Homo sapiens (Human)
生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.02-0.1 ng/mL.
性质
Recombinant
内毒素水平
<0.01 EU/ug
纯度
>95% as determined by SDS-PAGE.
预测分子量
15.1 kDa
状态
Lyophilized
保存溶液
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interleukin-3 (IL3) is a ~17 kDa protein. Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB.
1. Guba, SC. et al. (1989) The Journal of clinical investigation 84, 1701-6. PMID: 2556442
2. Okayama, Y. et al. (1993) Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 23, 901-10. PMID: 10779277
3. Hong, L. et al. (2020) Cytokine 136, 155268. PMID: 32889153
4. Luo, XJ. et al. (2012) PloS one 7, e50375. PMID: 23226269
5. Khapli, SM. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 171, 142-51. PMID: 12816992
