InVivoMAb Anti-Human IL-2 Antibody (Iv0020) (HX011010)

Detects IL2 in indirect ELISAs.

Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # HX011010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human IL2 (Iv0020).

Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL2 (Catalog No: HX011012)
Lane 2: negative control

Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.