Anti-Human PPIA Recombinant Nanobody (SAA1329) (HX128013)

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50ug
100ug
1mg

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概述
图片
参考文献
产品说明书

概述

货号

HX128013

品牌

abinScience

描述

Anti-Human PPIA Nanobody (SAA1329) [SAA1329] (HX128013) is a alpaca monoclonal antibody detecting PPIA in ELISA, WB. Suitable for Human.

种属反应性

Human

应用

ELISA, WB

宿主

Alpaca

克隆类型

Monoclonal

同种型

VHH-8His-Cys-tag

克隆号

SAA1329

靶标

PPIase A, Cyclosporin A-binding protein, Peptidyl-prolyl cis-trans isomerase A, Cyclophilin A, PPIA, CYPA, Rotamase A

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P62937

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Peptidyl-prolyl cis-trans isomerase A (PPIA) is a ~18 kDa protein. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2.

1. Liu, J. et al. (1991) Biochemistry 30, 2306-10. PMID: 2001362

2. Davis, TL. et al. (2010) PLoS biology 8, e1000439. PMID: 20676357

3. Song, F. et al. (2011) The Journal of biological chemistry 286, 8197-8203. PMID: 21245143

4. Foster, TL. et al. (2011) Journal of virology 85, 7460-4. PMID: 21593166

5. Lauranzano, E. et al. (2015) Brain : a journal of neurology 138, 974-91. PMID: 25678563

6. Yurchenko, V. et al. (2002) The Journal of biological chemistry 277, 22959-65. PMID: 11943775

7. Jin, ZG. et al. (2004) Arteriosclerosis, thrombosis, and vascular biology 24, 1186-91. PMID: 15130913

8. Xie, Y. et al. (2019) Cellular signalling 61, 57-65. PMID: 31063815

9. Wei, Y. et al. (2013) Inflammation 36, 567-72. PMID: 23180369

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human PPIA Nanobody (SAA1329)

参考文献

产品说明书

Anti-Human PPIA Recombinant Nanobody (SAA1329).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PPIA Recombinant Nanobody (SAA1329) (HX128013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PPIA Recombinant Nanobody (SAA1329) (HX128013)

概述

图片

参考文献

产品说明书

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