Anti-Human MMP13 Recombinant Antibody (40E09#) (HW650013)

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规格：

50ug
100ug
1mg

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概述
图片
参考文献
产品说明书

HW650013

abinScience

种属反应性

Human

ELISA, FCM

VHH-mFc (IgG2a)

40E09#

MMP-13, MMP13, Matrix metalloproteinase-13, Collagenase 3

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P45452

Anti-Human MMP13 Antibody (40E09#) (abinScience Cat# HW650013, RRID:AB_3729066)
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Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Collagenase 3 (MMP13) is a ~53 kDa protein. Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification.

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Bioactivity

Detects Human MMP13 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human MMP13 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP13 antibody monoclonal antibody (Catalog # HW650013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human MMP13 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP13 antibody monoclonal antibody (Catalog # HW650013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human MMP13 Antibody (40E09#).

产品说明书

Anti-Human MMP13 Recombinant Antibody (40E09#).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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实验相关试剂

Anti-Human MMP13 Recombinant Antibody (40E09#) (HW650013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MMP13 Recombinant Antibody (40E09#) (HW650013)

概述

图片

参考文献

产品说明书

相关产品推荐

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