Anti-Human CD31/PECAM1 Recombinant Antibody (SAA2179) (HB004033)

价格:

规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HB004033

abinScience

种属反应性

Human

ELISA, WB, FCM

VHH-mFc (IgG2a)

SAA2179

经测试应用

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P16284

Anti-Human CD31/PECAM1 Antibody (SAA2179) (abinScience Cat# HB004033, RRID:AB_3729544)

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Platelet endothelial cell adhesion molecule (CD31/PECAM1) is a ~82 kDa protein. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal.

1. Sachs, UJ. et al. (2007) The Journal of biological chemistry 282, 23603-12. PMID: 17580308

2. Dasgupta, B. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 5041-51. PMID: 19342684

3. Hu, M. et al. (2016) Scientific reports 6, 38655. PMID: 27958302

4. Brown, S. et al. (2002) Nature 418, 200-3. PMID: 12110892

5. Yeh, JC. et al. (2008) Biochemistry 47, 9029-39. PMID: 18672896

For research use only.

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

Flow cytometry

Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD31 monoclonal antibody (Catalog HB004033, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (Catalog # HB004033 , Blue Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (AntibodySystem: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD31/PECAM1 Antibody (SAA2179)

产品说明书

Anti-Human CD31/PECAM1 Recombinant Antibody (SAA2179).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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实验相关试剂

Anti-Human CD31/PECAM1 Recombinant Antibody (SAA2179) (HB004033)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD31/PECAM1 Recombinant Antibody (SAA2179) (HB004033)

概述

图片

参考文献

产品说明书

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