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Anti-G protein Recombinant Nanobody (Nb35) (HC114013)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HC114013
品牌abinScience
描述
Anti-G protein Nanobody (Nb35) [Nb35] (HC114013) is a alpaca monoclonal antibody detecting G protein in Cyro-EM, X-ray Crystallography. Suitable for Human.
种属反应性Human
应用Cyro-EM, X-ray Crystallography
宿主Alpaca
同种型VHH-His
克隆号Nb35
克隆类型Monoclonal
靶标G protein
内毒素水平<0.01 EU/ug
纯度>95% as determined by SDS-PAGE.
纯化方式Purified by Nickel column.
Accession号P63092
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Nanobody 35, Nanobody-35, Nb-35
背景

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short is a ~45 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins.

1. Pak, Y. et al. (2002) Molecular and cellular biology 22, 7942-52. PMID: 12391161
2. Gao, X. et al. (2007) The Journal of biological chemistry 282, 294-302. PMID: 17110384
3. Thiele, S. et al. (2011) Human mutation 32, 653-60. PMID: 21488135
4. Brand, CS. et al. (2015) Molecular pharmacology 88, 758-67. PMID: 26206488
5. Farfel, Z. et al. (1996) The Journal of biological chemistry 271, 19653-5. PMID: 8702665
6. Warner, DR. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 4268-72. PMID: 10200251
NoteFor research use only
图片
  • SDS-PAGE

    SDS-PAGE for Anti-G protein Nanobody (Nb35)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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