Bulk Anti-DYKDDDDK Tag (M2-ICH) Antibody - Mouse IgG1 (ICH1212G1-5mg)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

This is a recombinant version of the widely used M2 anti-DYKDDDDK (FLAG) monoclonal antibody, a mouse IgG1 raised against the DYKDDDDK epitope tag. Because DYKDDDDK is a synthetic peptide tag fused to recombinant proteins rather than a native antigen, this reagent is species-independent and works with tagged constructs expressed in any host system. Recombinant production gives consistent, animal-component-reduced lots with defined heavy- and light-chain sequences, minimizing the batch-to-batch variability of hybridoma material. It supports an unusually broad application range: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification when conjugated to agarose or magnetic beads, and use as an isotype control. Supplied at research grade with low endotoxin and available in bulk milligram-to-gram quantities, it is well suited to high-throughput tag detection, affinity capture, and scale-up purification workflows. For research use only (RUO); not for diagnostic or therapeutic use. DYKDDDDK (the FLAG tag) is an eight-residue hydrophilic peptide epitope (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) engineered as a fusion tag for the detection and purification of recombinant proteins. It is not a naturally occurring antigen; it is appended to the N- or C-terminus of a protein of interest via molecular cloning. Its short length and highly charged, solvent-exposed character mean it rarely perturbs the folding, localization, or function of the fusion partner, making it one of the most popular affinity and epitope tags. The terminal sequence also encodes an enterokinase cleavage site (DDDDK), allowing enzymatic removal of an N-terminal tag after purification. Antibodies against DYKDDDDK enable sensitive, sequence-specific recognition of tagged proteins across expression systems, supporting detection, immunoprecipitation, co-immunoprecipitation, imaging, and single-step affinity purification independent of the target protein's own antigenicity.