Bulk Anti-DYKDDDDK Tag (M2-ICH) Antibody - Mouse IgG2a (ICH1212G2-10mg)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

This is a bulk-format monoclonal antibody directed against the DYKDDDDK (FLAG) peptide tag, supplied by ichor.bio as a research-use-only reagent. The M2-ICH designation refers to the classic M2-type anti-FLAG specificity, a mouse IgG2a monoclonal that recognises the FLAG octapeptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) regardless of its position at the N-terminus, C-terminus, or internal sites of a fusion protein. Because the FLAG tag is a synthetic epitope rather than a biological target, this antibody is used as a general-purpose tool for detecting, immunoprecipitating, and purifying recombinant FLAG-tagged proteins expressed in bacterial, insect, or mammalian systems. The bulk (mg-to-gram) format and RUO grade are aimed at investigators who need consistent, large-scale material for affinity capture, Western blotting, immunocytochemistry, or high-throughput pull-down workflows. It is not a therapeutic, not a biosimilar, and is not intended for clinical use; it is a detection/purification reagent rather than a functional in-vivo antibody, and endotoxin/format specifications should be confirmed against the product datasheet for the intended application. DYKDDDDK, commonly called the FLAG tag, is a short hydrophilic synthetic peptide epitope (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) engineered onto recombinant proteins to enable detection and purification. It is not a naturally occurring protein or gene product, so there is no endogenous biology, pathway, or disease association to describe; its value lies entirely in being a small, well-characterised handle that rarely interferes with the folding, localisation, or activity of the fusion partner. The M2-type specificity binds the FLAG sequence in a position-independent manner, allowing tagging at either terminus or at internal loops. The tag can also be removed by enterokinase, which cleaves after the terminal lysine, leaving a near-native protein. These properties make anti-FLAG monoclonals a cornerstone reagent for affinity chromatography, co-immunoprecipitation, and expression monitoring across essentially all model systems.