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Anti-Hypusine Recombinant Antibody (Hpu24) (YP587013)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号YP587013
品牌abinScience
种属反应性General
应用ELISA, IHC, WB
同种型IgG
克隆号Hpu24
靶标Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号PubChem: 65396
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
文献1. Shiba T,et al. Hypusine, a new amino acid occurring in bovine brain. Isolation and structural determination. Biochim Biophys Acta. 1971 Sep 21;244(3):523-31. [YP587013]
2. ark MH, et al. Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. J Biol Chem. 2018 Nov 30;293(48):18710-18718. [YP587013]
NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Hypusine antibody (Hpu24).

  • SDS-PAGE

    SDS-PAGE for Anti-Hypusine antibody (Hpu24).

  • Western blot

    Western blot analysis was performed using anti-EIF5A monoclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: Hela cell lysate
    Lane 3: 293T cell lysate
    Lane 4: NIH3T3 cell lysate
    Lane 5: Mouse pancears lysate
    Lane 7: Rat pancears lysate

  • Western blot

    Western blot analysis was performed using anti-Eif5a polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: K562 cell lysate
    Lane 4: PC12 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: MCF7 cell lysate
    Lane 7: 293T cell lysate

公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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