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HEK293T Human CD166/ALCAM Stable Cell Line (HC678818)

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规格：
  • 1 ml
数量：
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概述
货号HC678818
品牌abinScience
描述
HEK293T Human CD166/ALCAM Stable Cell Line (HC678818) is a stable cell line expressing CD166/ALCAM. Engineered for consistent, high-level target expression in research applications.
Highlights
  • ●Stable Expression — Engineered for consistent, high-level target expression.
Accession号Q13740
应用FCM
种属Human
靶标CD166/ALCAM
性能Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
稳定性和存储Frozen in liquid nitrogen.
规格1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景

CD166 antigen (ALCAM) is a ~65 kDa protein. Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation.

1. Hassan, NJ. et al. (2004) European journal of immunology 34, 930-40. PMID: 15048703
2. Ikeda, K. et al. (2004) The Journal of biological chemistry 279, 55315-23. PMID: 15496415
3. Zimmerman, AW. et al. (2006) Blood 107, 3212-20. PMID: 16352806
4. Iolyeva, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 978-90. PMID: 23169771
5. Escoda-Ferran, C. et al. (2014) FEBS letters 588, 2805-13. PMID: 24945728
6. Bowen, MA. et al. (1995) The Journal of experimental medicine 181, 2213-20. PMID: 7760007
7. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938
8. Xu, Z. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1207-1219. PMID: 29263213
9. Chitteti, BR. et al. (2014) Blood 124, 519-29. PMID: 24740813
图片

  • Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD166 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
产品说明书
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
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质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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HEK293T Human CD166/ALCAM Stable Cell Line

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