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Anti-Poliovirus type 1 VP0/Capsid protein VP0 Polyclonal Antibody (VK703014)

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概述
货号VK703014
品牌abinScience
种属反应性Poliovirus type 1 (PV1)
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Poliovirus type 1 VP0/Capsid protein VP0 (Pro122-Gln341).
靶标Genome polyprotein, P1, Capsid protein VP0
纯化方式Purified by antigen affinity column.
Accession号P03300
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Genome polyprotein (VP0/Capsid protein VP0) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

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1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218
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2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424
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3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086
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4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193
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5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529
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6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571
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NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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