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Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A) (VK557013)

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规格:
  • 50ug
  • 100ug
  • 1mg
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概述
货号VK557013
品牌abinScience
描述
Anti-Poliovirus Capsid protein VP3 Nanobody (7A) [7A] (VK557013) is a alpaca monoclonal antibody detecting Capsid protein VP3 in ELISA, Neutralization.
种属反应性Poliovirus type 1 (PV1)
应用ELISA, Neutralization
宿主Alpaca
同种型VHH-8His-Cys-tag
克隆号7A
克隆类型Monoclonal
靶标Genome polyprotein, Capsid protein VP3, Virion protein 3
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Purified by Nickel column.
Accession号P03300
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Genome polyprotein (Capsid protein VP3) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218
2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424
3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086
4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193
5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529
6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Poliovirus Capsid protein VP3 Nanobody (7A)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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