Anti-Human PTH1R Recombinant Nanobody (SAA0797) (HX947013)

价格: --

规格：

50ug
100ug
1mg

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HX947013

品牌

abinScience

描述

Anti-Human PTH1R Nanobody (SAA0797) [SAA0797] (HX947013) is a alpaca monoclonal antibody detecting PTH1R in ELISA, RIA. Suitable for Human.

种属反应性

Human

应用

ELISA, RIA, WB

宿主

Alpaca

克隆类型

Monoclonal

同种型

VHH-8His-Cys-tag

克隆号

SAA0797

经测试应用

WB, IF

靶标

PTH/PTHr receptor, Parathyroid hormone 1 receptor, PTHR1, PTHR, PTH1 receptor, PTH/PTHrP type I receptor, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

Q03431

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) is a ~66 kDa protein. G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity. PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH.

1. Grauschopf, U. et al. (2000) Biochemistry 39, 8878-87. PMID: 10913300

2. Pioszak, AA. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5034-9. PMID: 18375760

3. Pioszak, AA. et al. (2009) The Journal of biological chemistry 284, 28382-28391. PMID: 19674967

4. Shimomura-Kuroki, J. et al. (2017) Odontology 105, 150-154. PMID: 27160269

5. Zhao, LH. et al. (2019) Science (New York, N.Y.) 364, 148-153. PMID: 30975883

6. Kobayashi, K. et al. (2022) Molecular cell 82, 3468-3483.e5. PMID: 35932760

7. Schneider, H. et al. (1993) European journal of pharmacology 246, 149-55. PMID: 8397094

8. Pioszak, AA. et al. (2010) The Journal of biological chemistry 285, 12435-44. PMID: 20172855

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human PTH1R Nanobody (SAA0797)
Bioactivity

Detects recombinant PTH1R (Catalog No: HX947012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-PTH1R monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PTH1R (Catalog No: HX947012)
Immunofluorescence

Immunofluorescence staining of MCF7 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane .

参考文献

产品说明书

Anti-Human PTH1R Recombinant Nanobody (SAA0797).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PTH1R Recombinant Nanobody (SAA0797) (HX947013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PTH1R Recombinant Nanobody (SAA0797) (HX947013)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价