Recombinant Human PTH1R Protein, N-His (PX-P5901) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
Q03431
蛋白长度
Ala28-Gly188
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
20.84 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
PTH/PTHr receptor, Parathyroid hormone 1 receptor, PTHR1, PTHR, PTH1 receptor, PTH/PTHrP type I receptor, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor
背景
Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) is a ~66 kDa protein. G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity. PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH.
1. Grauschopf, U. et al. (2000) Biochemistry 39, 8878-87. PMID: 10913300
2. Pioszak, AA. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5034-9. PMID: 18375760
3. Pioszak, AA. et al. (2009) The Journal of biological chemistry 284, 28382-28391. PMID: 19674967
4. Shimomura-Kuroki, J. et al. (2017) Odontology 105, 150-154. PMID: 27160269
5. Zhao, LH. et al. (2019) Science (New York, N.Y.) 364, 148-153. PMID: 30975883
6. Kobayashi, K. et al. (2022) Molecular cell 82, 3468-3483.e5. PMID: 35932760
7. Schneider, H. et al. (1993) European journal of pharmacology 246, 149-55. PMID: 8397094
8. Pioszak, AA. et al. (2010) The Journal of biological chemistry 285, 12435-44. PMID: 20172855
