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Anti-OGA Polyclonal Antibody (PTX19893)

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概述
货号PTX19893
品牌ProteoGenix
描述
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human OGA (Ser6-Lys240).
靶标OGA,Protein O-GlcNAcase,Meningioma-expressed antigen 5,Beta-N-acetylhexosaminidase,HEXC,N-acetyl-beta-glucosaminidase,KIAA0679,Nuclear cytoplasmic O-GlcNAcase and acetyltransferase,MEA5,MGEA5,Beta-hexosaminidase,NCOAT,Beta-N-acetylglucosaminidase,N-acetyl-beta-D-glucosaminidase
纯化方式Purified by antigen affinity column.
Accession号O60502
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Protein O-GlcNAcase (OGA) is a ~102 kDa protein. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.

1. Gao, Y. et al. (2001) The Journal of biological chemistry 276, 9838-45. PMID: 11148210
2. Wells, L. et al. (2002) The Journal of biological chemistry 277, 1755-61. PMID: 11788610
3. Li, J. et al. (2010) Biochemistry. Biokhimiia 75, 938-43. PMID: 20673219
4. Schimpl, M. et al. (2012) Chemistry & biology 19, 173-8. PMID: 22365600
5. Rao, FV. et al. (2013) Open biology 3, 130021. PMID: 24088714
6. Li, B. et al. (2017) Nature communications 8, 666. PMID: 28939839
7. Yu, F. et al. (2024) Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73, 5-17. PMID: 37962578
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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