Recombinant Human OGA Protein, N-His (PX-P5834) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
O60502
蛋白长度
Ser6-Lys240
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
28.59 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Protein O-GlcNAcase (OGA) is a ~102 kDa protein. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.
1. Gao, Y. et al. (2001) The Journal of biological chemistry 276, 9838-45. PMID: 11148210
2. Wells, L. et al. (2002) The Journal of biological chemistry 277, 1755-61. PMID: 11788610
3. Li, J. et al. (2010) Biochemistry. Biokhimiia 75, 938-43. PMID: 20673219
4. Schimpl, M. et al. (2012) Chemistry & biology 19, 173-8. PMID: 22365600
5. Rao, FV. et al. (2013) Open biology 3, 130021. PMID: 24088714
6. Li, B. et al. (2017) Nature communications 8, 666. PMID: 28939839
7. Yu, F. et al. (2024) Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73, 5-17. PMID: 37962578
