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Anti-Mycobacterium tuberculosis icl1/Isocitrate lyase 1 Recombinant Antibody (SAA2568) (JN930023)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号JN930023
品牌abinScience
种属反应性Mycobacterium tuberculosis
应用ELISA, WB
宿主Human
克隆类型Monoclonal
同种型VHH-hFc
克隆号SAA2568
靶标EC:4.1.3.1, ICL, Isocitrase, Isocitratase, Isocitrate lyase, icl
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P9WKK7
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Isocitrate lyase (icl1/Isocitrate lyase 1) is a ~47 kDa protein. Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It could also catalyze the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).

1. Sharma, V. et al. (2000) Nature structural biology 7, 663-8. PMID: 10932251
2. McKinney, JD. et al. (2000) Nature 406, 735-8. PMID: 10963599
3. Kumar, R. et al. (2008) Proteins 72, 892-900. PMID: 18275086
4. Moynihan, MM. et al. (2014) Biochemistry 53, 178-87. PMID: 24354272
NoteFor research use only
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Mycobacterium tuberculosis icl1/Isocitrate lyase 1 Antibody (SAA2568)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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