Anti-Human Desmoglein-3 Recombinant Antibody (DF151) (HW575107)

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100ug

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货号	HW575107
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG1, kappa
克隆号	DF151
经测试应用	WB, FCM
靶标	Cadherin family member 6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, CDHF6
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P32926
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Desmoglein-3 (DSG3) is a ~107 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle. Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells. May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3. 1. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537 2. Koch, PJ. et al. (1998) Journal of cell science 111 ( Pt 17), 2529-37. PMID: 9701552 3. Tsang, SM. et al. (2012) The Journal of pathology 227, 81-93. PMID: 22294297 4. Tsang, SM. et al. (2012) Experimental cell research 318, 2269-83. PMID: 22796473 5. Rötzer, V. et al. (2016) The Journal of investigative dermatology 136, 301-10. PMID: 26763450
Note	For research use only.

Bioactivity

Detects DSG3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human DSG3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DSG3 antibody monoclonal antibody (Catalog # HW575107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-human DSG3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human DSG3 (Catalog No: HW575011)
Lane 2: negative control

产品说明书

Anti-Human Desmoglein-3 Recombinant Antibody (DF151).pdf

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Anti-Human Desmoglein-3 Recombinant Antibody (DF151) (HW575107)

概述

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参考文献

产品说明书

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重组蛋白

抗体

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实验相关试剂

Anti-Human Desmoglein-3 Recombinant Antibody (DF151) (HW575107)

概述

图片

参考文献

产品说明书

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