Human Desmoglein-3 Recombinant Protein (C-His) (HW575011)
概述
图片
参考文献
产品说明书
概述
货号
HW575011
品牌
abinScience
描述
Recombinant Human DSG3 Protein, C-His (HW575011) expressed in Insect Cells, spanning Glu50-Arg615. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Insect cells
Accession号
P32926
蛋白长度
Glu50-Arg615
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
经测试应用
WB
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
64.95 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Cadherin family member 6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, CDHF6
背景
Desmoglein-3 (DSG3) is a ~107 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle. Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells. May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3.
1. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537
2. Koch, PJ. et al. (1998) Journal of cell science 111 ( Pt 17), 2529-37. PMID: 9701552
3. Tsang, SM. et al. (2012) The Journal of pathology 227, 81-93. PMID: 22294297
4. Tsang, SM. et al. (2012) Experimental cell research 318, 2269-83. PMID: 22796473
5. Rötzer, V. et al. (2016) The Journal of investigative dermatology 136, 301-10. PMID: 26763450
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human DSG3 Protein, C-His
Bioactivity
Detects Human DSG3 Antibody (DF151) in indirect ELISAs.
Western blot
Detected by Anti-DSG3 monoclonal antibody (Catalog No: HW575107)
