Anti-Human IL-1 alpha/IL-1F1 Recombinant Antibody (SAA0366) (HF802107)

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100ug

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货号	HF802107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM
同种型	IgG1, kappa
克隆号	SAA0366
经测试应用	FCM
靶标	IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P01583
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Interleukin-1 alpha (IL1A/IL1F1) is a ~30 kDa protein. Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. 1. Cohen, I. et al. (2015) Scientific reports 5, 14756. PMID: 26439902 2. Brikos, C. et al. (2007) Molecular & cellular proteomics : MCP 6, 1551-9. PMID: 17507369 3. Mosley, B. et al. (1987) The Journal of biological chemistry 262, 2941-4. PMID: 2950091 4. Wu, T. et al. (2004) Cytokine 25, 11-20. PMID: 14687581 5. Spiekstra, SW. et al. (2005) Experimental dermatology 14, 109-16. PMID: 15679580
Note	For research use only.

Flow-cytometry

Flow-cytometry using anti-human IL1A antibody.IL1A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IL1A antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IL1A antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A monoclonal antibody (Catalog HF802107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human IL-1 alpha/IL-1F1 Recombinant Antibody (SAA0366).pdf

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Anti-Human IL-1 alpha/IL-1F1 Recombinant Antibody (SAA0366) (HF802107)

概述

图片

参考文献

产品说明书

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实验相关试剂

Anti-Human IL-1 alpha/IL-1F1 Recombinant Antibody (SAA0366) (HF802107)

概述

图片

参考文献

产品说明书

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