Anti-Human IL-13 Recombinant Antibody (SAA0382) (HW632107)

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100ug

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货号	HW632107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, WB
同种型	IgG1, kappa
克隆号	SAA0382
经测试应用	WB, FCM
靶标	IL13, NC30, Interleukin-13, IL-13
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P35225
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Interleukin-13 (IL13) is a ~15 kDa protein. Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells. Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1. Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B. 1. Minty, A. et al. (1993) Nature 362, 248-50. PMID: 8096327 2. McKenzie, AN. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 3735-9. PMID: 8097324 3. Defrance, T. et al. (1994) The Journal of experimental medicine 179, 135-43. PMID: 7903680 4. Luttmann, W. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 1678-83. PMID: 8759755 5. Schnyder, B. et al. (1996) Blood 87, 4286-95. PMID: 8639787 6. Miloux, B. et al. (1997) FEBS letters 401, 163-6. PMID: 9013879 7. Kasaian, MT. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 187, 561-9. PMID: 21622864
Note	For research use only.

Bioactivity

Detects Human IL13 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL13 antibody.IL13 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL13 antibody monoclonal antibody (Catalog # HW632107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-IL13 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL13 (Catalog No: HW632012)
Lane 2: negative control

产品说明书

Anti-Human IL-13 Recombinant Antibody (SAA0382).pdf

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Anti-Human IL-13 Recombinant Antibody (SAA0382) (HW632107)

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Anti-Human IL-13 Recombinant Antibody (SAA0382) (HW632107)

概述

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参考文献

产品说明书

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