Anti-Human Podocalyxin Like Recombinant Antibody (Mab60) (HT033207)

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货号	HT033207
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, IHC, Neutralization
同种型	IgG2a, kappa
克隆号	Mab60
靶标	PCLP1, Podocalyxin-like protein 1, GCTM-2 antigen, Gp200, PODXL, Podocalyxin, PC, PCLP, PCLP-1
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	O00592
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Podocalyxin (PODXL) is a ~58 kDa protein. Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis.
Note	For research use only.

Western blot

Western blot analysis was performed using anti-PODXL monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PODXL (Catalog No: HT033012)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human PODXL antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PODXL monoclonal antibody (Catalog HT033207, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human Podocalyxin Like Recombinant Antibody (Mab60).pdf

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Anti-Human Podocalyxin Like Recombinant Antibody (Mab60) (HT033207)

概述

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参考文献

产品说明书

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Anti-Human Podocalyxin Like Recombinant Antibody (Mab60) (HT033207)

概述

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参考文献

产品说明书

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