Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO) (HW443013)

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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概述
图片
参考文献
产品说明书

概述

货号

HW443013

品牌

abinScience

描述

Anti-Human CD50/ICAM3 (ICM3) (HW443013) is a research-grade recombinant antibody targeting CD50. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

种属反应性

Human

应用

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

同种型

IgG1

克隆号

ICM3

表达系统

Mammalian cells

靶标

CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3

内毒素水平

< 10 EU/mg

纯度

>95% purity as determined by SDS-PAGE.

纯化方式

Protein A/G purified from cell culture supernatant.

Accession号

P32942

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Intercellular adhesion molecule 3 (CD50/ICAM3) is a ~59 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages. Is also a ligand for integrin ITGAD/ITGB2. Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells.

1. Fawcett, J. et al. (1992) Nature 360, 481-4. PMID: 1448173

2. Vazeux, R. et al. (1992) Nature 360, 485-8. PMID: 1448174

3. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590

4. Juan, M. et al. (1993) European journal of immunology 23, 1508-12. PMID: 8325327

5. de Fougerolles, AR. et al. (1993) The Journal of experimental medicine 177, 1187-92. PMID: 8459213

6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714

7. Geijtenbeek, TB. et al. (2000) Cell 100, 575-85. PMID: 10721994

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

图片

Bioactivity

Detects Human CD50/ICAM3 in indirect ELISAs.
Bioactivity

SEC-HPLC detection for Research Grade Anti-Human CD50/ICAM3 (ICM3).
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD50/ICAM3 (ICM3)
Flow CytoMetry

Flow-cytometry using APC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD50 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献

产品说明书

Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO) (HW443013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO) (HW443013)

概述

图片

参考文献

产品说明书

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