Anti-Human CD157 Reference Antibody (MEN1112, RUO) (HC654016)

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货号	HC654016
品牌	abinScience
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主	Human
同种型	IgG
克隆号	MEN1112
生物活性	MEN1112 specifically binds to Bst1/CD157-positive cell lines with an EC 50 of 1 nM, and does not bind to Bst1/CD157-negative cell lines [1]. MEN1112 binds to recombinant Bst1/CD157 antigen with an EC 50 of 0.4 nM [1]. MEN1112 exhibits stronger high-affinity binding to both high- and low-affinity alleles of the CD16A Fc receptor on effector cells due to its defucosylated Fc domain [1]. MEN1112 induces slow internalization of the Bst1/CD157 antigen on target cells, a property that helps enhance its ADCC efficacy [1]. MEN1112 potently mediates antibody-dependent cell-mediated cytotoxicity (ADCC) against U937 acute myeloid leukemia (AML) cells, with an EC 50 of 30-140 pM [3]. MEN1112-mediated target cell lysis (20 h post-administration) is significantly enhanced upon the addition of 5% healthy donor serum to the co-culture system of healthy monocytes and autologous NK cells [3].
表达系统	Mammalian cells
克隆类型	Monoclonal
靶标	ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Bone marrow stromal antigen 1, BST-1, ADP-ribosyl cyclase 2, CD157, Cyclic ADP-ribose hydrolase 2, BST1, cADPr hydrolase 2
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q10588
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	MEN1112
背景	ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 (CD157/BST1) is a ~35 kDa protein. Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable). 1. Hirata, Y. et al. (1994) FEBS letters 356, 244-8. PMID: 7805847
文献	1. Aud D, et al. MEN1112, a Novel Humanized De-Fucosylated Monoclonal Antibody with High Affinity and Specificity for Bst1/CD157 Antigen and Enhanced CD16 Binding. Blood. 2014 Dec 6;124(21):3607. [HC654016] 2. Venditti A, et al. MEN1112/OBT357, an Anti Bst1/CD157 Humanized Antibody Inducing Acute Myelogenous Leukemia (AML) Blast Depletion in an Autologous Ex Vivo Assay: A Potential New Targeted Therapy for AML. Blood. 2015;126(23):788. [HC654016] 3. Krupka C, et al. Targeting AML Using an Fc-Engineered BST1/CD157 Monoclonal Antibody. Blood. 2014 Dec 6;124(21):987. [HC654016]
Note	For research use only. Not suitable for clinical or therapeutic use.

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD157/BST1 (MEN1112)

产品说明书

Anti-Human CD157 Reference Antibody (MEN1112, RUO).pdf

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Anti-Human CD157 Reference Antibody (MEN1112, RUO) (HC654016)

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Anti-Human CD157 Reference Antibody (MEN1112, RUO) (HC654016)

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