Anti-Human CDCP1 Reference Antibody (RG7287, RUO) (HV451016)

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货号	HV451016
品牌	abinScience
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型	IgG1-kappa
克隆号	RG7287
表达系统	Mammalian cells
靶标	CD318, Transmembrane and associated with src kinases, TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1, CDCP1
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q9H5V8
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	RG7287
背景	CUB domain-containing protein 1 (CD318/CDCP1) is a ~92 kDa protein. May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
文献	1. Gwendlyn Kollmorgen, et al. Antibody mediated CDCP1 degradation as mode of action for cancer targeted therapy. Mol Oncol. 2013 Dec;7(6):1142-51. [HV451016]
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity

Detects CD318 in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD318/CDCP1 (RG7287).
Flow CytoMetry

Flow-cytometry using APC anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human CD318 antibody. HT29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD318 monoclonal antibody (Catalog HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CDCP1 Reference Antibody (RG7287, RUO).pdf

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Anti-Human CDCP1 Reference Antibody (RG7287, RUO) (HV451016)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CDCP1 Reference Antibody (RG7287, RUO) (HV451016)

概述

图片

参考文献

产品说明书

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