| 货号 | VK794012 |
|---|---|
| 品牌 | abinScience |
| 描述 | 3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.<br>Activity definition: 1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 μg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C. |
| 表达系统 | E. coli |
| 蛋白长度 | 3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli. |
| 应用 | Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP). |
| 种属 | HRV-14 |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 预测分子量 | 47.46 kDa |
| 状态 | Liquid |
| 保存溶液 | 50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Note | For research use only. |
SDS-PAGE for 3C Protease
M: Marker
1: Control
2: Fusion protein digest with 3C protease
| Case study | Formula | |
|---|---|---|
| concentration | 1 mg/ml | c mg/ml |
| volume | 100 µl | V ml |
| substrate | 100 µg | cV mg |
| 10X Buffer | 11 µl | V/9 ml |
| 3C Protease | 1 µl | cV/100 ml |
Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.
7X24小时产品咨询热线：027--87433958(9:00-18:00)，或扫描下方产品专员二维码或在线留言
武汉东湖新技术开发区神墩四路666号研发楼B区3层东
24小时产品查询
扫一扫关注我们
专属渠道经理
