3C Protease (VK794012)

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概述

货号	VK794012
品牌	abinScience
描述	3C Protease (VK794012) expressed in E. coli. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
蛋白长度	3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
应用	Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
状态	Liquid
保存溶液	50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for 3C Protease

Bioactivity

M: Marker
1: Control
2: Fusion protein digest with 3C protease

Case study		Formula
concentration	1 mg/ml	c mg/ml
volume	100 µl	V ml
substrate	100 µg	cV mg
10X Buffer	11 µl	V/9 ml
3C Protease	1 µl	cV/100 ml

Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.

参考文献

产品说明书

3C Protease.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

3C Protease (VK794012)

概述

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重组蛋白

抗体

试剂盒

实验相关试剂

3C Protease (VK794012)

概述

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参考文献

产品说明书

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