Human BTLA/CD272 Recombinant Protein (C-His) (HV375011)
概述
图片
参考文献
产品说明书
概述
货号
HV375011
品牌
abinScience
描述
Recombinant Human CD272/BTLA Protein, C-His (HV375011) expressed in Mammalian Cells, spanning Met1-Ser150.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
表达系统
Mammalian cells
Accession号
Q7Z6A9
蛋白长度
Met1-Ser150
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>85% as determined by SDS-PAGE.
预测分子量
18.22 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
B- and T-lymphocyte attenuator, BTLA, CD272, B- and T-lymphocyte-associated protein
背景
B- and T-lymphocyte attenuator (CD272/BTLA) is a ~32 kDa protein. Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells.
1. Watanabe, N. et al. (2003) Nature immunology 4, 670-9. PMID: 12796776
2. Gavrieli, M. et al. (2003) Biochemical and biophysical research communications 312, 1236-43. PMID: 14652006
3. Sedy, JR. et al. (2005) Nature immunology 6, 90-8. PMID: 15568026
4. Cai, G. et al. (2008) Nature immunology 9, 176-85. PMID: 18193050
5. Cheung, TC. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 183, 7286-96. PMID: 19915044
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human CD272/BTLA Protein, C-His
Bioactivity
Detected by Research Grade Anti-Human CD272/BTLA (ANB032) (Cat No.: HV375036) in indirect ELISA.
Bioactivity
Immobilized HV375011 Human CD272/BTLA Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind MV480011 Mouse CD270/TNFRSF14 Recombinant Protein (C-Fc), the EC 50 is 13.1 ng/mL.
