Recombinant Human HSP90B1 Protein, N-His (PX-P4788)
概述
图片
参考文献
概述
货号
PX-P4788
品牌
ProteoGenix
描述
Recombinant Human HSP90B1 Protein, N-His (PX-P4788) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
P14625
蛋白长度
Asp22-Ala311
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
34.93 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Endoplasmin (HSP90B1/GRP94) is a ~92 kDa protein. ATP-dependent chaperone involved in the processing of proteins in the endoplasmic reticulum, regulating their transport. Together with MESD, acts as a modulator of the Wnt pathway by promoting the folding of LRP6, a coreceptor of the canonical Wnt pathway. When associated with CNPY3, required for proper folding of Toll-like receptors. Promotes folding and trafficking of TLR4 to the cell surface. May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10.
1. Liu, B. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 6877-82. PMID: 23572575
2. Ma, M. et al. (2024) Science (New York, N.Y.) 386, 667-672. PMID: 39509507
3. Randow, F. et al. (2001) Nature cell biology 3, 891-6. PMID: 11584270
4. Zhang, M. et al. (2020) Cell 181, 637-652.e15. PMID: 32272059
