Human ALCAM/CD166 Recombinant Protein (C-Fc) (HC678021)
概述
图片
参考文献
产品说明书
概述
货号
HC678021
品牌
abinScience
描述
Recombinant Human CD166/ALCAM Protein, C-Fc (HC678021) expressed in Mammalian Cells, spanning Met1-Ala526. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
Q13740
蛋白长度
Met1-Ala526
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
85.18 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CD166 antigen is a ~65 kDa protein. Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation.
1. Hassan, NJ. et al. (2004) European journal of immunology 34, 930-40. PMID: 15048703
2. Ikeda, K. et al. (2004) The Journal of biological chemistry 279, 55315-23. PMID: 15496415
4. Iolyeva, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 978-90. PMID: 23169771
5. Escoda-Ferran, C. et al. (2014) FEBS letters 588, 2805-13. PMID: 24945728
6. Bowen, MA. et al. (1995) The Journal of experimental medicine 181, 2213-20. PMID: 7760007
7. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938
8. Xu, Z. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1207-1219. PMID: 29263213
Immobilized HB797021 Human CD6 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HC678021 Human CD166/ALCAM Recombinant Protein (C-Fc), the EC 50 is 54.9 ng/mL.
