ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
37.42 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Ceramide synthase 2 is a ~44 kDa protein. Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively. Plays a non-redundant role in the synthesis of ceramides with very-long-chain fatty acids in kidney, liver and brain. Regulates the abundance of myelin-specific sphingolipids galactosylceramide and sulfatide that affects myelin sheath architecture and motor neuron functions.
1. Lahiri, S. et al. (2007) FEBS letters 581, 5289-94. PMID: 17977534
2. Laviad, EL. et al. (2008) The Journal of biological chemistry 283, 5677-84. PMID: 18165233
3. Mizutani, Y. et al. (2008) Journal of lipid research 49, 2356-64. PMID: 18541923
4. Spassieva, SD. et al. (2009) The Biochemical journal 424, 273-83. PMID: 19728861
5. Ohno, Y. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18439-44. PMID: 20937905
6. Tidhar, R. et al. (2012) The Journal of biological chemistry 287, 3197-206. PMID: 22144673
7. Kim, HJ. et al. (2012) Journal of lipid research 53, 1701-7. PMID: 22661289
8. Sassa, T. et al. (2016) The Journal of biological chemistry 291, 7477-87. PMID: 26887952
9. Tidhar, R. et al. (2018) The Journal of biological chemistry 293, 9912-9921. PMID: 29632068
