Anti-Human Desmoglein-3 Recombinant Antibody (AK23) (HW575013)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HW575013

abinScience

种属反应性

Human

IP, IF, ELISA

Mouse

Monoclonal

IgG2a, kappa

经测试应用

Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6, DSG3, CDHF6

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P32926

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Desmoglein-3 (DSG3) is a ~107 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle. Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells. May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3.

1. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537

2. Koch, PJ. et al. (1998) Journal of cell science 111 ( Pt 17), 2529-37. PMID: 9701552

3. Tsang, SM. et al. (2012) The Journal of pathology 227, 81-93. PMID: 22294297

4. Tsang, SM. et al. (2012) Experimental cell research 318, 2269-83. PMID: 22796473

5. Rötzer, V. et al. (2016) The Journal of investigative dermatology 136, 301-10. PMID: 26763450

For research use only

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

SDS-PAGE

SDS-PAGE for Anti-Human DSG3 Antibody (AK23)
Flow cytometry

Flow-cytometry using anti-human Desmoglein 3 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Desmoglein 3 monoclonal antibody (Catalog # HW575013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SEC-HPLC

SEC-HPLC detection for Anti-Human DSG3 Antibody (AK23).

产品说明书

Anti-Human Desmoglein-3 Recombinant Antibody (AK23).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Desmoglein-3 Recombinant Antibody (AK23) (HW575013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Desmoglein-3 Recombinant Antibody (AK23) (HW575013)

概述

图片

参考文献

产品说明书

相关产品推荐

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