Human Glypican 3 Recombinant Protein (N-His) (HW310022)

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50ug
100ug
1mg

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概述

货号	HW310022
品牌	abinScience
描述	Recombinant Human GPC3 Protein, N-His (HW310022) expressed in E. coli, spanning Gly69-Ser413. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
Accession号	P51654
蛋白长度	Gly69-Ser413
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Homo sapiens (Human)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	41.95 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	GPC3, GTR2-2, Glypican-3, Glypican-3 alpha subunit, Glypican-3 beta subunit, Intestinal protein OCI-5, MXR7, OCI5
背景	Glypican-3 is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063 2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623 3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449 4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790
Note	For research use only

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参考文献

产品说明书

Human Glypican 3 Recombinant Protein (N-His).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Human Glypican 3 Recombinant Protein (N-His) (HW310022)

概述

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参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Human Glypican 3 Recombinant Protein (N-His) (HW310022)

概述

图片

参考文献

产品说明书

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