| 货号 | HW310023 | ||||||
|---|---|---|---|---|---|---|---|
| 品牌 | abinScience | ||||||
| 种属反应性 | Human, Mouse | ||||||
| 应用 | ELISA, FCM, WB | ||||||
| 宿主 | Human | ||||||
| 同种型 | IgG1, kappa | ||||||
| 克隆号 | YP7 | ||||||
| 克隆类型 | Monoclonal | ||||||
| 靶标 | GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5 | ||||||
| 内毒素水平 | Please contact with the lab for this information. | ||||||
| 纯度 | >95% as determined by SDS-PAGE. | ||||||
| 纯化方式 | Protein A/G purified from cell culture supernatant. | ||||||
| Accession号 | P51654 | ||||||
| 状态 | Liquid | ||||||
| 保存溶液 | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Note | For research use only. |
Detects Human GPC3 in indirect ELISAs.
Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human GPC3 Antibody (YP7).
Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310023) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-GPC3/Glypican 3 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant mouse GPC3/Glypican 3 (Catalog No: MW310011)
Lane 2: negative control
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