Anti-Human Glypican 3 Recombinant Antibody (YP7) (HW310023)

价格:

规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HW310023

abinScience

种属反应性

Human, Mouse

ELISA, FCM, WB

IgG1, kappa

经测试应用

WB, FCM

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P51654

Anti-Human GPC3 Antibody (YP7) (abinScience Cat# HW310023, RRID:AB_3727765)
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Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

For research use only.

Isotype Control

Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107)

Bioactivity

Detects Human GPC3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human GPC3 Antibody (YP7).
Flow CytoMetry

Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310023) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-GPC3/Glypican 3 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant mouse GPC3/Glypican 3 (Catalog No: MW310011)
Lane 2: negative control
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate

产品说明书

Anti-Human Glypican 3 Recombinant Antibody (YP7).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

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实验相关试剂

Anti-Human Glypican 3 Recombinant Antibody (YP7) (HW310023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Glypican 3 Recombinant Antibody (YP7) (HW310023)

概述

图片

参考文献

产品说明书

相关产品推荐

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